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Establishment and application of multiplex real-time PCR for simultaneous detection of four viruses associated with porcine reproductive failure

9 Feb 2023

Abstract excerpt

Many pathogens cause reproductive failure in sows suffering a broad spectrum of sequelae, including abortions, stillbirth, mummification, embryonic death, and infertility. Although various detection methods, such as polymerase chain reaction (PCR) and real-time PCR, have been widely used for molecular diagnosis, mainly for a single pathogen. In this study, we developed a multiplex real-time PCR method for the...

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PMID: 36846754
DOI: 10.3389/fmicb.2023.1092273

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