Article
Enhancing genetic mapping of complex genomes through the design of highly-multiplexed SNP arrays: application to the large and unsequenced genomes of white spruce and black spruce.
BMC genomics - 18 Jan 2008
Pavy Nathalie, Pelgas Betty, Beauseigle Stéphanie, Blais Sylvie, Gagnon France, Gosselin Isabelle, Lamothe Manuel, Isabel Nathalie, Bousquet Jean
Abstract excerpt
BACKGROUND: To explore the potential value of high-throughput genotyping assays in the analysis of large and complex genomes, we designed two highly multiplexed Illumina bead arrays using the GoldenGate SNP assay for gene mapping in white spruce (Picea glauca [Moench] Voss) and black spruce (Picea mariana [Mill.] B.S.P.). RESULTS: Each array included 768 SNPs, identified by resequencing genomic DNA from parents...
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