Article
A genome-wide SNP genotyping resource for tropical pine tree species.
Molecular ecology resources - 1 Feb 2022
Jackson Colin, Christie Nanette, Reynolds Sharon Melissa, Marais Gerhard C, Tii-Kuzu Yokateme, Caballero Madison, Kampman Tamanique, Visser Erik A, Naidoo Sanushka, Kain Dominic, Whetten Ross W, Isik Fikret, Wegrzyn Jill, Hodge Gary R, Acosta Juan J, Myburg Alexander A
Abstract excerpt
We performed gene and genome targeted SNP discovery towards the development of a genome-wide, multispecies genotyping array for tropical pines. Pooled RNA-seq data from shoots of seedlings from five tropical pine species was used to identify transcript-based SNPs resulting in 1.3 million candidate Affymetrix SNP probe sets. In addition, we used a custom 40 K probe set to perform capture-seq in pooled DNA from 81...
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