Article
An Efficient Genotyping Method in Chicken Based on Genome Reducing and Sequencing.
PloS one - 1 Jan 2015
Liao Rongrong, Wang Zhen, Chen Qiang, Tu Yingying, Chen Zhenliang, Wang Qishan, Yang Changsuo, Zhang Xiangzhe, Pan Yuchun
Abstract excerpt
Single nucleotide polymorphisms (SNPs) are essential for identifying the genetic mechanisms of complex traits. In the present study, we applied genotyping by genome reducing and sequencing (GGRS) method to construct a 252-plex sequencing library for SNP discovery and genotyping in chicken. The library was successfully sequenced on an Illumina HiSeq 2500 sequencer with a paired-end pattern; approximately 400...
Topics
- Animals
- Chickens
- Cost-Benefit Analysis
- Genomics
- Genotyping Techniques
- High-Throughput Nucleotide Sequencing
- Oligonucleotide Array Sequence Analysis
- Polymorphism, Single Nucleotide
- Quality Control
- Sequence Analysis, DNA