Article
Genotyping of classical swine fever virus using high-resolution melt analysis.
Journal of virological methods - 1 Nov 2015
Titov Ilya, Tsybanov Sodnom, Malogolovkin Alexander
Abstract excerpt
Discrimination between different field and vaccine strains of classical swine fever virus (CSFV) is crucial for meaningful disease diagnosis and epidemiological investigation. In this study, a rapid method for differentiating vaccine strains and outbreak CSFV isolates by combined RT-PCR and high-resolution melt (HRM) analysis has been developed. The assay is based on PCR amplification of short fragments from the...
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