Article
Genetic characterization of E2 gene of classical swine fever virus by restriction fragment length polymorphism and phylogenetic analysis.
Virus genes - 1 Jun 2010
Chen Ning, Li Dejiang, Yuan Xuemei, Li Xiaoliang, Hu Hongxia, Zhu Binglin, Wan Xiaoyuan, Fang Weihuan
Abstract excerpt
An RT-nested PCR (RT-nPCR)-based restriction fragment length polymorphism (RFLP) analyses of the E2 gene were developed for genetic subtyping and differentiation of vaccinated and infected classical swine fever virus (CSFV) strains. RT-nPCR identified 96 CSFV-positive samples from 321 clinical specimens from southeastern China during 2003-2008. The PCR products of positive samples were further differentiated...
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