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Article

Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB).

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] - 1 Jun 2015

Jung Lenice Roteia Cardoso, Bomfim Maria Rosa Quaresma, Kroon Erna Geessien, Nunes Álvaro Cantini

Abstract excerpt

Leptospires are usually classified by methods based on DNA-DNA hybridization and the conventional cross-agglutination absorption test, which uses polyclonal antibodies against lipopolysaccharides. In this study, the amplification of the rpoB gene, which encodes the beta-subunit of RNA polymerase, was used as an alternative tool to identify Leptospira. DNA extracts from sixty-eight serovars were obtained, and the...

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Identifiers

PMID: 26273261
DOI: 10.1590/S1517-838246220120018
PMCID: PMC4507538

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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