rpoB Gene Analysis as a Novel Strategy for Identification of Spirochetes from the Genera Borrelia , Treponema , and Leptospira

Abstract excerpt

Spirochetes are emerging pathogens for which culture and identification are partly unresolved. In fact, 16S rRNA-based sequencing is by far the most widely used PCR methodology that is able to detect such uncultivable pathogens. However, this assay actually has some limitations linked to potential problems of contamination, which hampers diagnosis. To circumvent this, we have devised a simple PCR strategy...

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