Article
Molecular analysis of Leptospira spp. isolated from humans by restriction fragment length polymorphism, real-time PCR and pulsed-field gel electrophoresis.
FEMS microbiology letters - 1 Nov 2009
Turk Nenad, Milas Zoran, Mojcec Vesna, Ruzic-Sabljic Eva, Staresina Vilim, Stritof Zrinka, Habus Josipa, Postic Daniele
Abstract excerpt
A total of 17 Leptospira clinical strains isolated from humans in Croatia were serologically and genetically analysed. For serovar identification, the microscopic agglutination test (MAT) and pulsed-field gel electrophoresis (PFGE) were used. To identify isolates on genomic species level, PCR-based restriction fragment length polymorphism (RFLP) and real-time PCR were performed. MAT revealed the following...
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