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Polymerase chain reaction for detection of Leptospira spp. in clinical samples

1 Sept 1992

Abstract excerpt

A sensitive assay for Leptospira spp., the causative agent of leptospirosis, was developed on the basis of the polymerase chain reaction (PCR). A 331-bp sequence from the Leptospira interrogans serovar canicola rrs (16S) gene was amplified, and the PCR products were analyzed by DNA-DNA hybridization by using a 289-bp fragment internal to the amplified DNA. Specific PCR products also were obtained with DNA from...

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PMID: 1400983
DOI: 10.1128/jcm.30.9.2219-2224.1992

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