Article
Molecular characterization of the rpoB gene in Brucella species: new potential molecular markers for genotyping.
Microbes and infection - 1 Mar 2006
Marianelli Cinzia, Ciuchini Franco, Tarantino Michela, Pasquali Paolo, Adone Rosanna
Abstract excerpt
The rpoB gene encoding the beta subunit of the DNA-dependent RNA polymerase was molecularly characterized by PCR amplification and DNA sequencing in 26 Brucella reference strains by using primers selected according to the B. melitensis 16 M rpoB published sequence. Comparison of the rpoB nucleotide sequence of all Brucella strains analysed revealed specific nucleotide variations associated with different Brucella...
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