Article
Catalysis of GTP hydrolysis by small GTPases at atomic detail by integration of X-ray crystallography, experimental, and theoretical IR spectroscopy.
The Journal of biological chemistry - 2 Oct 2015
Rudack Till, Jenrich Sarah, Brucker Sven, Vetter Ingrid R, Gerwert Klaus, Kötting Carsten
Abstract excerpt
Small GTPases regulate key processes in cells. Malfunction of their GTPase reaction by mutations is involved in severe diseases. Here, we compare the GTPase reaction of the slower hydrolyzing GTPase Ran with Ras. By combination of time-resolved FTIR difference spectroscopy and QM/MM simulations we elucidate that the Mg(2+) coordination by the phosphate groups, which varies largely among the x-ray structures, is...
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