Article
Generation of MANAbodies specific to HLA-restricted epitopes encoded by somatically mutated genes.
Proceedings of the National Academy of Sciences of the United States of America - 11 Aug 2015
Skora Andrew D, Douglass Jacqueline, Hwang Michael S, Tam Ada J, Blosser Richard L, Gabelli Sandra B, Cao Jianhong, Diaz Luis A, Papadopoulos Nickolas, Kinzler Kenneth W, Vogelstein Bert, Zhou Shibin
Abstract excerpt
Mutant epitopes encoded by cancer genes are virtually always located in the interior of cells, making them invisible to conventional antibodies. We here describe an approach to identify single-chain variable fragments (scFvs) specific for mutant peptides presented on the cell surface by HLA molecules. We demonstrate that these scFvs can be successfully converted to full-length antibodies, termed MANAbodies,...
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