Article
Do major histocompatibility complex tag single nucleotide polymorphisms accurately identify HLA-B27 in the Turkish population?
International journal of rheumatic diseases - 1 Dec 2017
Akar Servet, Igci Yusuf Z, Sari Ismail, Pala Elif, Geyik Esra, Tas Mehmet N, Solmaz Dilek, Çetin Pinar, Akkoc Nurullah
Abstract excerpt
OBJECTIVE: To evaluate the performance of human leukocyte antigen (HLA)-B27 tag single nucleotide polymorphisms (SNPs) by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). METHODS: We genotyped three SNPs (rs116488202, rs13202464 and rs4349859). The primers were designed using Primer3 algorithm via primer-BLAST interface. PCR products were digested by using NlaIII, BmrI and TaqαI...
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