Article
Passenger Mutations Confound Interpretation of All Genetically Modified Congenic Mice.
Immunity - 21 Jul 2015
Vanden Berghe Tom, Hulpiau Paco, Martens Liesbet, Vandenbroucke Roosmarijn E, Van Wonterghem Elien, Perry Seth W, Bruggeman Inge, Divert Tatyana, Choi Sze Men, Vuylsteke Marnik, Shestopalov Valery I, Libert Claude, Vandenabeele Peter
Abstract excerpt
Targeted mutagenesis in mice is a powerful tool for functional analysis of genes. However, genetic variation between embryonic stem cells (ESCs) used for targeting (previously almost exclusively 129-derived) and recipient strains (often C57BL/6J) typically results in congenic mice in which the targeted gene is flanked by ESC-derived passenger DNA potentially containing mutations. Comparative genomic analysis of...
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