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Article

Global Methylation and Hydroxymethylation in DNA from Blood and Saliva in Healthy Volunteers

1 Jan 2015

Abstract excerpt

Aims. We describe a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify and compare simultaneously global methylation and hydroxymethylation in human DNA of different tissues. Materials and Methods. Blood and saliva DNA from fourteen volunteers was processed for epigenetic endpoints using LC-MS/MS and PCR-pyrosequencing technology. Results. Global DNA methylation was significantly lower...

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PMID: 26090450
DOI: 10.1155/2015/845041

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