Article
[High-resolution melting technology for detecting genetic polymorphisms of IL-1β and IL-1Ra in patients with coronary artery disease].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Jun 2015
Qi Xiaoming, Bai Feng, You Chongge, Guo Xinling, Shi Lei, Hu Zhibin
Abstract excerpt
OBJECTIVE: To develop a method of high-resolution melting curve analysis (HRM) for investigating the association of single nucleotide polymorphisms (SNPs) of IL-1β and IL-1Ra genes with susceptibility to coronary heart disease (CHD) and serological indexes in CHD. METHODS: PCR-HRM assays were established for detecting four SNPs (IL-1B-511C>T, IL-1B-31C>T, IL-1B+3954C>T and IL-1RNT>C) in 260 patients with CHD and...
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