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Article

The folding unit of phosphofructokinase-2 as defined by the biophysical properties of a monomeric mutant.

Biophysical journal - 5 May 2015

Ramírez-Sarmiento César A, Baez Mauricio, Zamora Ricardo A, Balasubramaniam Deepa, Babul Jorge, Komives Elizabeth A, Guixé Victoria

Abstract excerpt

Escherichia coli phosphofructokinase-2 (Pfk-2) is an obligate homodimer that follows a highly cooperative three-state folding mechanism N2 ↔ 2I ↔ 2U. The strong coupling between dissociation and unfolding is a consequence of the structural features of its interface: a bimolecular domain formed by intertwining of the small domain of each subunit into a flattened β-barrel. Although isolated monomers of E. coli...

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Identifiers

PMID: 25954892
DOI: 10.1016/j.bpj.2015.04.001
PMCID: PMC4423062

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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