Article
Flow cytometric analysis of FSHR, BMRR1B, LHR and apoptosis in granulosa cells and ovulation rate in merino sheep.
Reproduction (Cambridge, England) - 1 Aug 2015
Regan Sheena L P, McFarlane James R, O'Shea Tim, Andronicos Nicholas, Arfuso Frank, Dharmarajan Arun, Almahbobi Ghanim
Abstract excerpt
The aim of the present study was to determine the direct cause of the mutation-induced, increased ovulation rate in Booroola Merino (BB) sheep. Granulosa cells were removed from antral follicles before ovulation and post-ovulation from BB (n=5) and WT (n=12) Merino ewes. Direct immunofluorescence measurement of mature cell surface receptors using flow cytometry demonstrated a significant up-regulation of FSH...
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