Article
Two rare novel polymorphisms in the D8S1179 and D13S317 markers and method to mitigate their impact on human identification.
Croatian medical journal - 1 Aug 2004
Forrest Shawn W, Kupferschmid Timothy D, Hendrickson Brant C, Judkins Thaddeus, Petersen Daniel J, Scholl Thomas
Abstract excerpt
AIM: Two rare polymorphisms were identified at the primer annealing sites of the short tandem repeat (STR) loci D8S1179 and D13S317 for a commercial multiplex STR system commonly used for human identification. These polymorphisms resulted in weak amplification from the affected alleles. Therefore, alternative polymerase chain reaction (PCR) thermal cycling conditions were developed that promoted more even signal...
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