Article
A protocol for direct and rapid multiplex PCR amplification on forensically relevant samples.
Forensic science international. Genetics - 1 Mar 2012
Verheij Saskia, Harteveld Joyce, Sijen Titia
Abstract excerpt
Forensic DNA typing involves a multi-step workflow. Steps include DNA isolation, quantification, amplification of a set of short tandem repeat (STR) markers, separation of polymerase chain reaction (PCR) products by capillary electrophoresis (CE) and DNA profile analysis and interpretation. With that, the process takes around 10-12h. For several scenarios it may be very valuable to speed up this process and...
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