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Article

The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse.

Journal of bacteriology - 1 Apr 2008

Durfee Tim, Nelson Richard, Baldwin Schuyler, Plunkett Guy, Burland Valerie, Mau Bob, Petrosino Joseph F, Qin Xiang, Muzny Donna M, Ayele Mulu, Gibbs Richard A, Csörgo Bálint, Pósfai György, Weinstock George M, Blattner Frederick R

Abstract excerpt

Escherichia coli DH10B was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids. The strain was constructed by serial genetic recombination steps, but the underlying sequence changes remained unverified. We report the complete genomic sequence of DH10B by using reads...

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Identifiers

PMID: 18245285
DOI: 10.1128/JB.01695-07
PMCID: PMC2293198

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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