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Article

Enzymatic characterization of recombinant nitrate reductase expressed and purified from Neurospora crassa.

Fungal genetics and biology : FG & B - 1 Jul 2015

Ringel Phillip, Probst Corinna, Dammeyer Thorben, Buchmeier Sabine, Jänsch Lothar, Wissing Josef, Tinnefeld Philip, Mendel Ralf R, Jockusch Brigitte M, Kruse Tobias

Abstract excerpt

We established an expression and purification procedure for recombinant protein production in Neurospora crassa (N. crassa). This Strep-tag® based system was successfully used for purifying recombinant N. crassa nitrate reductase (NR), whose enzymatic activity was compared to recombinant N. crassa NR purified from Escherichia coli. The purity of the two different NR preparations was similar but NR purified from...

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PMID: 25914160
DOI: 10.1016/j.fgb.2015.04.016

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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