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Article

Expression and purification of the transcription factor NtcA from the cyanobacterium Anabaena PCC 7120.

Protein expression and purification - 1 Dec 1999

Wisén S, Jiang F, Bergman B, Mannervik B

Abstract excerpt

The transcription factor NtcA from the cyanobacterium Anabaena PCC 7120 was heterologously expressed in Escherichia coli. In order to optimize the expression of NtcA, random silent mutations were introduced at the 5' end of the DNA encoding the protein. To get as high a yield of pure protein as possible, different strategies of expression as well as purification conditions were used. Under optimal expression...

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Identifiers

PMID: 10600452
DOI: 10.1006/prep.1999.1131

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Expression and purification of the transcription factor NtcA from the cyanobacterium Anabaena PCC 7120.PMID 10600452Read ↗

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