Article
Purification and in vitro activities of the Bacillus subtilis TnrA transcription factor.
Journal of molecular biology - 30 Jun 2000
Wray L V, Zalieckas J M, Fisher S H
Abstract excerpt
The Bacillus subtilis nitrogen regulatory protein TnrA was purified and its interaction with the nrgAB regulatory region examined. The TnrA protein activates transcription from the nrgAB promoter in vitro. DNase I footprinting and methylation protection experiments demonstrated that TnrA binds to an inverted repeat, upstream of the -35 region of the nrgAB promoter. Gel mobility retardation assays were used to...
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