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Cell‐free eukaryotic systems for the production, engineering, and modification of scFv antibody fragments

22 May 2014

Abstract excerpt

Open cell‐free translation systems based on Escherichia coli cell lysates have successfully been used to produce antibodies and antibody fragments. In this study, we demonstrate the cell‐free expression of functional single‐chain antibody variable fragments (scFvs) in a eukaryotic and endotoxin‐free in vitro translation system based on Spodoptera frugiperda ( Sf 21) insect cell extracts. Three scFv candidates...

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PMID: 25821419
DOI: 10.1002/elsc.201400036

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