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Post‐Translational Modifications of Histones in Human Sperm

24 Mar 2015

Abstract excerpt

We examined the levels and distribution of post-translationally modified histones and protamines in human sperm. Using western blot immunoassay, immunofluorescence, mass spectrometry (MS), and FLIM-FRET approaches, we analyzed the status of histone modifications and the protamine P2. Among individual samples, we observed variability in the levels of H3K9me1, H3K9me2, H3K27me3, H3K36me3, and H3K79me1, but the...

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PMID: 25808548
DOI: 10.1002/jcb.25170

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