Article
Universal Primers for Detection and Sequencing of Hepatitis B Virus Genomes across Genotypes A to G.
Journal of clinical microbiology - 1 Jun 2015
Chook Jack Bee, Teo Woon Li, Ngeow Yun Fong, Tee Kok Keng, Ng Kee Peng, Mohamed Rosmawati
Abstract excerpt
Hepatitis B virus (HBV) has been divided into 10 genotypes, A to J, based on an 8% nucleotide sequence divergence between genotypes. The conventional practice of using a single set of primers to amplify a near-complete HBV genome is hampered by its low analytical sensitivity. The current practice of using overlapping conserved primer sets to amplify a complete HBV genome in a clinical sample is limited by the...
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