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miRNA in situ hybridization in circulating tumor cells - MishCTC

17 Mar 2015

Abstract excerpt

Circulating tumor cells (CTCs) must be phenotypically and genetically characterized before they can be utilized in clinical applications. Here, we present the first protocol for the detection of miRNAs in CTCs using in situ hybridization (ISH) combined with immunomagnetic selection based on cytokeratin (CK) expression and immunocytochemistry. Locked-Nucleic Acid (LNA) probes associated with an enzyme-labeled...

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PMID: 25777797
DOI: 10.1038/srep09207

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