A new live-cell biobank workflow efficiently recovers heterogeneous melanoma cells from native biopsies.

Abstract excerpt

Fibroblast contamination can make establishing primary melanoma cell cultures from native biopsies a major challenge, due to fibroblasts overgrowing the melanoma cells. Standard protocols therefore enrich for highly proliferative melanoma cells that grow well in vitro but may not represent the full range of in vivo tumor heterogeneity. Here we apply conditional methods that more effectively retrieve melanoma...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Biological Specimen Banks
• Share to Biopsy