Article
Alpha-carboxy nucleoside phosphonates as universal nucleoside triphosphate mimics.
Proceedings of the National Academy of Sciences of the United States of America - 17 Mar 2015
Balzarini Jan, Das Kalyan, Bernatchez Jean A, Martinez Sergio E, Ngure Marianne, Keane Sarah, Ford Alan, Maguire Nuala, Mullins Niki, John Jubi, Kim Youngju, Dehaen Wim, Vande Voorde Johan, Liekens Sandra, Naesens Lieve, Götte Matthias, Maguire Anita R, Arnold Eddy
Abstract excerpt
Polymerases have a structurally highly conserved negatively charged amino acid motif that is strictly required for Mg(2+) cation-dependent catalytic incorporation of (d)NTP nucleotides into nucleic acids. Based on these characteristics, a nucleoside monophosphonate scaffold, α-carboxy nucleoside phosphonate (α-CNP), was designed that is recognized by a variety of polymerases. Kinetic, biochemical, and...
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