Article
Monitoring F1651 P-like fimbria expression at the single-cell level reveals a highly heterogeneous phenotype.
Infection and immunity - 1 May 2015
Graveline Richard, Lavoie Rémi, Garneau Philippe, Daigle France, Sénéchal Serge, Martin Christine, Harel Josée
Abstract excerpt
F1651 and the pyelonephritis-associated pili (Pap) are two members of the type P family of adhesive factors. They play a key role in establishing disease caused by extraintestinal pathogenic Escherichia coli (ExPEC) strains in animals and humans. Both F1651 and Pap are under the control of an epigenetic and reversible switch that defines the number of fimbriated (ON) and afimbriated (OFF) cells within a clonal...
Topics
- Artificial Gene Fusion
- Escherichia coli
- Fimbriae Proteins
- Fimbriae, Bacterial
- Flow Cytometry
- Gene Expression Profiling
- Genes, Reporter
- Green Fluorescent Proteins
- Microscopy, Fluorescence
- Phenotype