Article
Less is more: extreme genome complexity reduction with ddRAD using Ion Torrent semiconductor technology.
Molecular ecology resources - 1 Sept 2015
Pukk Lilian, Ahmad Freed, Hasan Shihab, Kisand Veljo, Gross Riho, Vasemägi Anti
Abstract excerpt
Massively parallel sequencing a small proportion of the whole genome at high coverage enables answering a wide range of questions from molecular evolution and evolutionary biology to animal and plant breeding and forensics. In this study, we describe the development of restriction-site associated DNA (RAD) sequencing approach for Ion Torrent PGM platform. Our protocol results in extreme genome complexity...
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