Article
Continuous xylanase production with Aspergillus nidulans under pyridoxine limitation using a trickle bed reactor.
Bioresource technology - 1 Jan 2015
Müller Michael, Prade Rolf A, Segato Fernando, Atiyeh Hasan K, Wilkins Mark R
Abstract excerpt
A trickle bed reactor (TBR) with recycle was designed and tested using Aspergillus nidulans with a pyridoxine marker and over-expressing/secreting recombinant client xylanase B (XynB). The pyridoxine marker prevented the fungus from synthesizing its own pyridoxine and fungus was unable to grow when no pyridoxine was present in the medium; however, enzyme production was unaffected. Uncontrolled mycelia growth that...
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