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Article

Isolation of myofibroblasts from mouse and human esophagus.

Journal of visualized experiments : JoVE - 18 Jan 2015

Gargus Matthew, Niu Chao, Shaker Anisa

Abstract excerpt

Murine and human esophageal myofibroblasts are generated via enzymatic digestion. Neonate (8-12 day old) murine esophagus is harvested, minced, washed, and subjected to enzymatic digestion with collagenase and dispase for 25 min. Human esophageal resection specimens are stripped of muscularis propria and adventitia and the remaining mucosa is minced, and subjected to enzymatic digestion with collagenase and...

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Identifiers

PMID: 25650889
DOI: 10.3791/52215
PMCID: PMC4354535

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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