Article
Establishment of mouse intestinal myofibroblast cell lines.
World journal of gastroenterology - 7 May 2013
Kawasaki Hideyoshi, Ohama Takashi, Hori Masatoshi, Sato Koichi
Abstract excerpt
AIM: To establish novel intestinal myofibroblast (IMF) cell lines from mouse colonic mucosa and investigate their biological characters. METHODS: Primary IMFs were isolated from mucosal tissues of mouse colon that was denuded of epithelial cells and smooth muscle layer. For immortalization, primary IMFs were transfected with simian virus 40 large T antigen (designated as LmcMF). We also isolated some primary IMFs...
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