Article
[Application value of allele frequencies in direct identification of ambiguous HLA genotypes].
Zhongguo shi yan xue ye xue za zhi - 1 Apr 2009
Cheng Liang-Hong, Zou Hong-Yan, Li Zhen, Jin Shi-Zheng, Wang Da-Min, Gao Su-Qin
Abstract excerpt
This study was aimed to investigate the application value of allele frequencies in direct identification of the ambiguous HLA genotypes. The HLA-A, HLA-B and HLADRB1 loci in 658 Chinese Han donor were detected by PCR-SBT method, the ambiguous genotyping samples were identified by using high resolution PCR-SSP and heterozygous ambiguity resolution primers (HAPRs) methods. The relative probability of true genotypes...
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