Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Fractionation of human spermatogenic cells using STA-PUT gravity sedimentation and their miRNA profiling.

Scientific reports - 30 Jan 2015

Liu Yun, Niu Minghui, Yao Chencheng, Hai Yanan, Yuan Qingqing, Liu Yang, Guo Ying, Li Zheng, He Zuping

Abstract excerpt

Human spermatogenic cells have not yet been isolated, and notably, their global miRNA profiles remain unknown. Here we have effectively isolated human spermatogonia, pachytene spermatocytes and round spermatids using STA-PUT velocity sedimentation. RT-PCR, immunocytochemistry and meiosis spread assays revealed that the purities of isolated human spermatogonia, pachytene spermatocytes, and round spermatids were...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Fractionation
Share to Cell Separation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Cell Shape

Share to Centrifugation

Share to Gravitation

Share to Spermatids

Share to Spermatocytes

Share to Spermatogonia

Source

Open in PubMed Open full text

Identifiers

PMID: 25634318
DOI: 10.1038/srep08084
PMCID: PMC5155379

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Fractionation of human spermatogenic cells using STA-PUT gravity sedimentation and their miRNA profiling.PMID 25634318Read ↗

Distinct Expression Profiles and Novel Targets of MicroRNAs in Human Spermatogonia, Pachytene Spermatocytes, and Round Spermatids between OA Patients and NOA PatientsSemantic proximity Read ↗

Unraveling transcriptome dynamics in human spermatogenesisSemantic proximity Read ↗

Isolation, characterization, and culture of human spermatogonia.Semantic proximity Read ↗

What has single-cell RNA-seq taught us about mammalian spermatogenesis?Semantic proximity Read ↗

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory EquipmentSemantic proximity Read ↗

Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human SpermatogenesisSemantic proximity Read ↗

Small RNA molecules in the regulation of spermatogenesisSemantic proximity Read ↗

RNA profiling of human testicular cells identifies syntenic lncRNAs associated with spermatogenesisSemantic proximity Read ↗

The thermo-sensitive gene expression signatures of spermatogenesisSemantic proximity Read ↗

Efficient generation of functional haploid spermatids from human germline stem cells by three-dimensional-induced systemSemantic proximity Read ↗

Molecular Signatures of Primary Human Spermatogonial Progenitors and Its Neighboring Peritubular Stromal Compartment.Semantic proximity Read ↗

Fluorescence- and magnetic-activated cell sorting strategies to isolate and enrich human spermatogonial stem cellsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.