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Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells

26 Jan 2015

Abstract excerpt

Linking the heavy chain (HC) and light chain (LC) genes required for monoclonal antibodies (mAb) production on a single cassette using 2A peptides allows control of LC and HC ratio and reduces non-expressing cells. Four 2A peptides derived from the foot-and-mouth disease virus (F2A), equine rhinitis A virus (E2A), porcine teschovirus-1 (P2A) and Thosea asigna virus (T2A), respectively, were compared for...

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PMID: 25621616
DOI: 10.1080/19420862.2015.1008351

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