Quantitative polymerase chain reaction analysis with allele-specific oligonucleotide primers for individual IgH VDJ regions to evaluate tumor burden in myeloma patients.

doi:10.1016/j.exphem.2015.01.002

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Community

Back to search

Article

Quantitative polymerase chain reaction analysis with allele-specific oligonucleotide primers for individual IgH VDJ regions to evaluate tumor burden in myeloma patients.

Experimental hematology - 1 May 2015

Sata Hiroshi, Shibayama Hirohiko, Maeda Ikuhiro, Habuchi Yoko, Nakatani Eiji, Fukushima Kentaro, Fujita Jiro, Ezoe Sachiko, Tadokoro Seiji, Maeda Tetsuo, Mizuki Masao, Kosugi Satoru, Nakagawa Masashi, Ueda Shuji, Iida Masato, Tokumine Yukihiro, Azenishi Yasuhiko, Mitsui Hideki, Oritani Kenji, Kanakura Yuzuru

Abstract excerpt

Quantitative polymerase chain reaction (PCR) with patient-specific, allele-specific oligonucleotide (ASO) primers for individual immunoglobulin H VDJ region (ASO-PCR) amplification was performed using several sources of clinical material, including mRNA from peripheral blood cells (PBMNCs), whole bone marrow cells (BMMNCs), and the CD20+ CD38- B-cell population in bone marrow, as well as cell-free DNA from the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to ADP-ribosyl Cyclase 1

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Antigens, CD20

Share to Bone Marrow Cells

Share to Immunoglobulin Heavy Chains

Share to Multiple Myeloma

Share to Neoplasm, Residual

Share to Oligonucleotides

Share to Tumor Burden

Source

Open in PubMed

Identifiers

PMID: 25591497
DOI: 10.1016/j.exphem.2015.01.002

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Quantitative polymerase chain reaction analysis with allele-specific oligonucleotide primers for individual IgH VDJ regions to evaluate tumor burden in myeloma patients.PMID 25591497Read ↗

Detection and quantitation of malignant cells in the peripheral blood of multiple myeloma patients.Semantic proximity Read ↗

Identification of clonotypic IgH VDJ sequences in multiple myeloma.Semantic proximity Read ↗

Quantitation of minimal residual disease in multiple myeloma using an allele-specific real-time PCR assay.Semantic proximity Read ↗

Kappa deleting element as an alternative molecular target for minimal residual disease assessment by real-time quantitative PCR in patients with multiple myeloma.Semantic proximity Read ↗

The bone marrow of multiple myeloma patients contains B cell populations at different stages of differentiation that are clonally related to the malignant plasma cell.Semantic proximity Read ↗

Prognostic value of deep sequencing method for minimal residual disease detection in multiple myelomaSemantic proximity Read ↗

Minimal residual disease monitoring in multiple myeloma: a comparison between allelic-specific oligonucleotide real-time quantitative polymerase chain reaction and flow cytometry.Semantic proximity Read ↗

Comparative analysis of minimal residual disease detection by multiparameter flow cytometry and enhanced ASO RQ-PCR in multiple myelomaSemantic proximity Read ↗

A High Frequency of Circulating B Cells Share Clonotypic Ig Heavy-Chain VDJ Rearrangements With Autologous Bone Marrow Plasma Cells in Multiple Myeloma, as Measured by Single-Cell and In Situ Reverse Transcriptase-Polymerase Chain ReactionSemantic proximity Read ↗

Real-time polymerase chain reaction of immunoglobulin rearrangements for quantitative evaluation of minimal residual disease in multiple myelomaSemantic proximity Read ↗

ELDA qASO-PCR for High Sensitivity Detection of Tumor Cells in Bone Marrow and Peripheral Blood.Semantic proximity Read ↗

Disruption of immunoglobulin heavy and light chain assembly by antisense oligonucleotides impairs protein homeostasis and myeloma cell survivalSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.