Article
Quantitation of minimal residual disease in multiple myeloma using an allele-specific real-time PCR assay.
Experimental hematology - 1 Sept 2000
Rasmussen T, Poulsen T S, Honoré L, Johnsen H E
Abstract excerpt
OBJECTIVE: To develop a real-time PCR method, based on the 5'nuclease TaqMan technology, for quantitation of clonal cells in multiple myeloma (MM). MATERIALS AND METHODS: The real-time quantitative PCR method incorporates both an allele-specific oligonucleotides (ASO) primer and an ASO dual-labeled fluorogenic probe (ASO TaqMan probe). The ASO primer and probe corresponded to the complementary determining region...
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