Article
Protein photochromism observed by ultrafast vibrational spectroscopy.
The journal of physical chemistry. B - 10 Oct 2013
Lukacs Andras, Haigney Allison, Brust Richard, Addison Kiri, Towrie Michael, Greetham Gregory M, Jones Garth A, Miyawaki Atsushi, Tonge Peter J, Meech Stephen R
Abstract excerpt
Photochromic proteins, such as Dronpa, are of particular importance in bioimaging and form the basis of ultraresolution fluorescence microscopy. The photochromic reaction involves switching between a weakly emissive neutral trans form of the chromophore (A) and its emissive cis anion (B). Controlling the rates of switching has the potential to significantly enhance the spatial and temporal resolution in...
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