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Article

Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants.

Biophysical journal - 15 Jun 2007

Ando Ryoko, Flors Cristina, Mizuno Hideaki, Hofkens Johan, Miyawaki Atsushi

Abstract excerpt

Dronpa absorbs blue light and emits bright green fluorescence. It can also be converted by strong irradiation at 490 nm to a nonfluorescent state, which can then be switched back to the original emissive state with irradiation at 400 nm. Through semirandom mutagenesis studies, we have developed two mutants of Dronpa that show efficient photoswitching kinetics. Compared to Dronpa, the mutants can be turned off by...

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Identifiers

PMID: 17384059
DOI: 10.1529/biophysj.107.105882
PMCID: PMC1877776

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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