Article
Fully automated sample preparation microsystem for genetic testing of hereditary hearing loss using two-color multiplex allele-specific PCR.
Analytical chemistry - 20 Jan 2015
Zhuang Bin, Gan Wupeng, Wang Shuaiqin, Han Junping, Xiang Guangxin, Li Cai-Xia, Sun Jing, Liu Peng
Abstract excerpt
A fully automated microsystem consisting of a disposable DNA extraction and PCR microchip, as well as a compact control instrument, has been successfully developed for genetic testing of hereditary hearing loss from human whole blood. DNA extraction and PCR were integrated into a single 15-μL reaction chamber, where a piece of filter paper was embedded for capturing genomic DNA, followed by in-situ PCR...
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