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Article

[Study on the impact of Trp1707Ser mutation on the binding mechanism of rF VIII light chain with VWF].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 1 Nov 2014

Chi Kun, Shao Yanyan, Lu Yeling, Dai Jing, Ding Qiulan, Wang Xuefeng, Wang Hongli

Abstract excerpt

OBJECTIVE: To disclose the impact of Trp1707Ser mutation on the binding mechanism of rFVIII light chain (rFVIII LC) with VWF. METHODS: Using long-chain PCR technique, we constructed rFVIII LC plasmids of both wild type and Trp1707Ser mutant type. BL21 competent cells were used for protein expression. Gradient renaturation was employed to refold protein. SDS-PAGE and Western blot were performed to identify the...

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Identifiers

PMID: 25417877
DOI: 10.3760/cma.j.issn.0253-2727.2014.11.010

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VWF
SDS
SPR

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