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Article

[Trans-splicing of Cys mutated coagulation factor VIII].

Yao xue xue bao = Acta pharmaceutica Sinica - 1 Jun 2012

Zhu Fu-Xiang, Liu Ze-Long, Miao Jing, Qu Hui-Ge, Chi Xiao-Yan

Abstract excerpt

To investigate the improving effect of inter-chain disulfide formation on protein trans-splicing, we introduce a Cys point mutation at Tyr(664) in heavy chain and at Thr(1826) in light chain of B-domain-deleted FVIII (BDD-FVIII). By co-transfection of COS-7 cell with the two Cys mutated chain genes, the intracellular protein splicing, inter-chain disulfide formation, secreted BDD-FVIII and bioactivity in culture...

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PMID: 22919720

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