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Article

Proteomic profiling of macrophages by 2D electrophoresis.

Journal of visualized experiments : JoVE - 4 Nov 2014

Bouvet Marion, Turkieh Annie, Acosta-Martin Adelina E, Chwastyniak Maggy, Beseme Olivia, Amouyel Philippe, Pinet Florence

Abstract excerpt

The goal of the two-dimensional (2D) electrophoresis protocol described here is to show how to analyse the phenotype of human cultured macrophages. The key role of macrophages has been shown in various pathological disorders such as inflammatory, immunological, and infectious diseases. In this protocol, we use primary cultures of human monocyte-derived macrophages that can be differentiated into the M1...

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Identifiers

PMID: 25408153
DOI: 10.3791/52219
PMCID: PMC4353417

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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