Article
Reduction of Powerplex(®) Y23 reaction volume for genotyping buccal cell samples on FTA(TM) cards.
Journal of forensic sciences - 1 Jan 2015
Raziel Aliza, Dell'Ariccia-Carmon Aviva, Zamir Ashira
Abstract excerpt
PowerPlex(®) Y23 is a novel kit for Y-STR typing that includes new highly discriminating loci. The Israel DNA Database laboratory has recently adopted it for routine Y-STR analysis. This study examined PCR amplification from 1.2-mm FTA punch in reduced volumes of 5 and 10 μL. Direct amplification and washing of the FTA punches were examined in different PCR cycle numbers. One short robotically performed wash was...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
