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Article

Design and development of novel single multiplex system incorporating 26 rapidly mutating Y-STRs; 26 RM Yplex.

Electrophoresis - 1 Oct 2022

Albastaki Abdulla, Naji Mohammed, Lootah Reem, Almheiri Reem, Almulla Hanan, Alreyami Afra, Almarri Iman, Yu Zailiang, Zhang Yueke, Shu Chang, Alghafri Rashed

Abstract excerpt

This article details the development of a single multiplex system amplifying 26 rapidly mutating Y-STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y-STRs commercial kits in differentiating closely related males. The multiplex performed well in the prevalidation tests and showed great potential...

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PMID: 35899438
DOI: 10.1002/elps.202200099

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