Article
An updated validation of Promega's PowerPlex 16 System: high throughput databasing under reduced PCR volume conditions on Applied Biosystem's 96 capillary 3730xl DNA Analyzer.
Journal of forensic sciences - 1 Nov 2008
Spathis Rita, Lum J Koji
Abstract excerpt
The PowerPlex 16 System from Promega Corporation allows single tube multiplex amplification of sixteen short tandem repeat (STR) loci including all 13 core combined DNA index system STRs. This report presents an updated validation of the PowerPlex 16 System on Applied Biosystem's 96 capillary 3730xl DNA Analyzer. The validation protocol developed in our laboratory allows for the analysis of 1536 loci (96 x 16) in...
Topics
- Alleles
- DNA Fingerprinting
- Female
- Humans
- Male
- Polymerase Chain Reaction
- Reproducibility of Results
- Tandem Repeat Sequences
